Hotspot

trnE-UUC-rpoB

LSC · 11583-12436 · 854 bp

Back to Jurinea

Pi 0.00937

MarkerSeek score 65.30

Primer available yes

Label trnE-UUC-rpoB

Feature Metadata

label_name: trnE-UUC-rpoB
feature_type: igs
region: LSC
length_bp: 854
variable_sites: 16
pi: 0.00937
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-rpoB_p1	GCATGATTTACCCCCAGAGG	AGTTCTTCTGTCAAACCCTGA	55.0	42.9	58.0	56.8	1014-1016 bp	1.0	63.4
trnE-UUC-rpoB_p2	GGCATGATTTACCCCCAGAG	AGTTCTTCTGTCAAACCCTGA	55.0	42.9	58.0	56.8	1015-1017 bp	1.0	63.4
trnE-UUC-rpoB_p3	AACCCTCGTTATCTCCGTGA	AGTTCTTCTGTCAAACCCTGA	50.0	42.9	58.2	56.8	988-990 bp	1.0	62.3
trnE-UUC-rpoB_p4	ACCCTCGTTATCTCCGTGAA	AGTTCTTCTGTCAAACCCTGA	50.0	42.9	58.2	56.8	987-989 bp	1.0	62.3
trnE-UUC-rpoB_p5	TGAACCCTCGTTATCTCCGT	AGTTCTTCTGTCAAACCCTGA	50.0	42.9	58.2	56.8	990-992 bp	1.0	62.3