Hotspot

trnS-GCU-trnC-GCA

LSC · 8523-9273 · 751 bp

Back to Jurinea

Pi 0.00466

MarkerSeek score 42.30

Primer available yes

Label trnS-GCU-trnC-GCA

Feature Metadata

label_name: trnS-GCU-trnC-GCA
feature_type: igs
region: LSC
length_bp: 751
variable_sites: 7
pi: 0.00466
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnC-GCA_p1	ACGGAAAGAGAGGGATTCGA	TCAGAGATTCTCGCGAGTCT	50.0	50.0	58.2	58.0	953-963 bp	1.0	79.1
trnS-GCU-trnC-GCA_p2	ACGGAAAGAGAGGGATTCGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.2	58.1	902-912 bp	1.0	78.7
trnS-GCU-trnC-GCA_p3	ACGGAAAGAGAGGGATTCGA	CACTCAGAGATTCTCGCGAG	50.0	55.0	58.2	57.9	956-966 bp	1.0	78.5
trnS-GCU-trnC-GCA_p4	ACGGAAAGAGAGGGATTCGA	ACTCAGAGATTCTCGCGAGT	50.0	50.0	58.2	58.3	955-965 bp	1.0	77.3
trnS-GCU-trnC-GCA_p5	ACGGAAAGAGAGGGATTCGA	AACACTCAGAGATTCTCGCG	50.0	50.0	58.2	57.7	958-968 bp	1.0	77.2