Hotspot

ycf1

Genome · 31067-38326 · 7260 bp

Back to Juniperus

Pi 0.0178

MarkerSeek score 51.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: Genome
length_bp: 7260
variable_sites: 372
pi: 0.0178
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CAGGGGTTGGAAAGGACAAA	TGCTGGACATAACGAAGCTC	50.0	50.0	57.9	58.0	847-1183 bp	1.0	91.3
ycf1_p2	CAGGGGTTGGAAAGGACAAA	AGTTCGTCCCTTCCTTCTGA	50.0	50.0	57.9	58.0	783-1119 bp	1.0	91.3
ycf1_p3	AAACAGGGGTTGGAAAGGAC	TGCTGGACATAACGAAGCTC	50.0	50.0	57.9	58.0	850-1186 bp	1.0	91.3
ycf1_p4	AAACAGGGGTTGGAAAGGAC	AGTTCGTCCCTTCCTTCTGA	50.0	50.0	57.9	58.0	786-1122 bp	1.0	91.3
ycf1_p5	TGGTGGTTAGGTGCAATCAC	TGCTGGACATAACGAAGCTC	50.0	50.0	58.1	58.0	1392-1728 bp	1.0	89.0