Hotspot

atpB-atpE

Genome · 126744-126753 · 10 bp

Back to Juniperus

Pi 0.0476

MarkerSeek score 77.10

Primer available yes

Label atpB-atpE

Feature Metadata

label_name: atpB-atpE
feature_type: igs
region: Genome
length_bp: 10
variable_sites: 1
pi: 0.0476
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-atpE_p1	GAAGCTACCGCAAAGGCTAT	ACAACTCGATTGGGAGTGAG	50.0	50.0	58.0	57.2	88-93 bp	1.0	78.1
atpB-atpE_p2	GAAGCTACCGCAAAGGCTAT	CAACTCGATTGGGAGTGAGT	50.0	50.0	58.0	57.2	87-92 bp	1.0	78.1
atpB-atpE_p3	ACCGCAAAGGCTATGAACTT	CAACTCGATTGGGAGTGAGT	45.0	50.0	57.8	57.2	81-86 bp	1.0	77.2
atpB-atpE_p4	TGGTCTACCCGAACAGTCTT	ACAACTCGATTGGGAGTGAG	50.0	50.0	58.0	57.2	131-136 bp	1.0	77.0
atpB-atpE_p5	TGGTCTACCCGAACAGTCTT	CAACTCGATTGGGAGTGAGT	50.0	50.0	58.0	57.2	130-135 bp	1.0	77.0