Hotspot

rrn16-trnI-GAU

Genome · 54628-55682 · 1055 bp

Back to Juniperus

Pi 0.0245
MarkerSeek score 61.30
Primer available yes
Label rrn16-trnI-GAU

Feature Metadata

label_name
rrn16-trnI-GAU
feature_type
igs
region
Genome
length_bp
1055
variable_sites
61
pi
0.0245
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.83
markerseek_score
61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rrn16-trnI-GAU_p1 AGTGACTGGAGTGAAGTCGT CTACCACTGAGCTAATAGCCC 50.0 52.4 58.3 57.3 821-1127 bp 1.0 73.0
rrn16-trnI-GAU_p2 AAGTCGTAACAAGGTAGCCG CTACCACTGAGCTAATAGCCC 50.0 52.4 57.6 57.3 808-1114 bp 1.0 72.6
rrn16-trnI-GAU_p3 AGTGACTGGAGTGAAGTCGT TACCACTGAGCTAATAGCCC 50.0 50.0 58.3 56.1 820-1126 bp 1.0 71.2
rrn16-trnI-GAU_p4 AAGTCGTAACAAGGTAGCCG TACCACTGAGCTAATAGCCC 50.0 50.0 57.6 56.1 807-1113 bp 1.0 70.9
rrn16-trnI-GAU_p5 GGCTAGTGACTGGAGTGAAG CTACCACTGAGCTAATAGCCC 55.0 52.4 57.3 57.3 825-1131 bp 1.0 70.1