Hotspot

trnV-GAC-rrn16

Genome · 52588-53143 · 556 bp

Back to Juniperus

Pi 0.0148

MarkerSeek score 60.60

Primer available yes

Label trnV-GAC-rrn16

Feature Metadata

label_name: trnV-GAC-rrn16
feature_type: igs
region: Genome
length_bp: 556
variable_sites: 24
pi: 0.0148
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-GAC-rrn16_p1	ACCTTGACGTGGTGAAAGTC	GGGCAGGTTCTTACGCATTA	50.0	50.0	58.1	58.0	719-810 bp	1.0	84.2
trnV-GAC-rrn16_p2	CCTTGACGTGGTGAAAGTCA	GGGCAGGTTCTTACGCATTA	50.0	50.0	58.1	58.0	718-809 bp	1.0	84.2
trnV-GAC-rrn16_p3	ACCTTGACGTGGTGAAAGTC	TACGCATTACTCACCCGTTC	50.0	50.0	58.1	57.7	708-799 bp	1.0	82.3
trnV-GAC-rrn16_p4	CCTTGACGTGGTGAAAGTCA	TACGCATTACTCACCCGTTC	50.0	50.0	58.1	57.7	707-798 bp	1.0	82.3
trnV-GAC-rrn16_p5	ACCTTGACGTGGTGAAAGTC	CTTACGCATTACTCACCCGT	50.0	50.0	58.1	57.7	710-801 bp	1.0	82.2