Hotspot

rps12-trnV-GAC

Genome · 51533-52515 · 983 bp

Back to Juniperus

Pi 0.0153

MarkerSeek score 68.60

Primer available yes

Label rps12-trnV-GAC

Feature Metadata

label_name: rps12-trnV-GAC
feature_type: igs
region: Genome
length_bp: 983
variable_sites: 41
pi: 0.0153
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-trnV-GAC_p1	ATCGTACTCTGGCGACTTTG	GACTTTCACCACGTCAAGGT	50.0	50.0	57.7	58.1	972-1265 bp	1.0	84.4
rps12-trnV-GAC_p2	ATCGTACTCTGGCGACTTTG	TGACTTTCACCACGTCAAGG	50.0	50.0	57.7	58.1	973-1266 bp	1.0	84.4
rps12-trnV-GAC_p3	GCGTAAGGCAGAGTTTGGTT	GACTTTCACCACGTCAAGGT	50.0	50.0	58.8	58.1	953-1246 bp	1.0	80.6
rps12-trnV-GAC_p4	GCGTAAGGCAGAGTTTGGTT	TGACTTTCACCACGTCAAGG	50.0	50.0	58.8	58.1	954-1247 bp	1.0	80.6
rps12-trnV-GAC_p5	ATCGTACTCTGGCGACTTTG	AGGTGACACTCTACCACTGA	50.0	50.0	57.7	57.3	956-1249 bp	1.0	79.4