Hotspot

ycf1-trnL-CAA

Genome · 38327-39436 · 1110 bp

Back to Juniperus

Pi 0.0327

MarkerSeek score 64.40

Primer available yes

Label ycf1-trnL-CAA

Feature Metadata

label_name: ycf1-trnL-CAA
feature_type: igs
region: Genome
length_bp: 1110
variable_sites: 108
pi: 0.0327
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.66
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-trnL-CAA_p1	CCTGAACCCACGGTATGATG	TACCATTCCACCATCAAGGC	55.0	50.0	58.0	57.6	546-1173 bp	1.0	88.1
ycf1-trnL-CAA_p2	CCTGAACCCACGGTATGATG	CGTGTCTACCATTCCACCAT	55.0	50.0	58.0	57.3	552-1179 bp	1.0	86.1
ycf1-trnL-CAA_p3	CCTGAACCCACGGTATGATG	GCGTGTCTACCATTCCACC	55.0	57.9	58.0	58.2	553-1180 bp	1.0	81.7
ycf1-trnL-CAA_p4	GATCCCTGAACCCACGGTAT	TACCATTCCACCATCAAGGC	55.0	50.0	58.9	57.6	550-1177 bp	1.0	81.5
ycf1-trnL-CAA_p5	CCTGAACCCACGGTATGATG	ACCATTCCACCATCAAGGC	55.0	52.6	58.0	57.7	545-1172 bp	1.0	81.3