Hotspot

rps12-clpP

Genome · 27968-28740 · 773 bp

Back to Juniperus

Pi 0.0503

MarkerSeek score 79.30

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: Genome
length_bp: 773
variable_sites: 110
pi: 0.0503
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	TTTCTTATCGGCTGTCTCGC	CGTGTAGAAGGAAAGCTCCC	50.0	55.0	58.1	58.0	1071-1716 bp	1.0	89.9
rps12-clpP_p2	GCATACTCCCTTCCGTTGAG	CCGTGTAGAAGGAAAGCTCC	55.0	55.0	58.1	58.0	1124-1769 bp	1.0	89.8
rps12-clpP_p3	GCATACTCCCTTCCGTTGAG	CCTCCGTGTAGAAGGAAAGC	55.0	55.0	58.1	58.0	1127-1772 bp	1.0	89.8
rps12-clpP_p4	GCATACTCCCTTCCGTTGAG	CGTGTAGAAGGAAAGCTCCC	55.0	55.0	58.1	58.0	1123-1768 bp	1.0	89.8
rps12-clpP_p5	GCATACTCCCTTCCGTTGAG	GTCCGCAATAGGCCCATAAA	55.0	50.0	58.1	58.0	1256-1901 bp	1.0	89.3