primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
accD_p1	accD	1	TCTATTCTAGCTCGCACCCA	TGGCAAATTCGTAACTAGACCA	20	22	50.0	40.9	57.9	57.7	0.0	0.0	2.341968	1771	4539	2823-2934	0.888889	64.7
accD_p2	accD	2	TGGGGTCAGTGGATCAGTAG	TGGCAAATTCGTAACTAGACCA	20	22	55.0	40.9	58.1	57.7	0.0	0.0	2.408587	1771	4539	2873-2984	0.888889	64.1
accD_p3	accD	3	GGGGTCAGTGGATCAGTAGA	TGGCAAATTCGTAACTAGACCA	20	22	55.0	40.9	57.8	57.7	0.0	0.0	2.435323	1771	4539	2872-2983	0.888889	63.9
accD_p4	accD	4	ATTCTAGCTCGCACCCAAAC	TGGCAAATTCGTAACTAGACCA	20	22	50.0	40.9	58.3	57.7	0.0	0.0	2.535536	1771	4539	2820-2931	0.888889	63.1
accD_p5	accD	5	ATGGGGTCAGTGGATCAGTA	TGGCAAATTCGTAACTAGACCA	20	22	50.0	40.9	57.4	57.7	0.0	0.0	2.843148	1771	4539	2874-2985	0.888889	60.6
accD-rpl23_p1	accD-rpl23	1	TTCTCGTATGTGCTTCTGGC	ACGATACAGACGCTTGTGAC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.031603	4540	5416	1731-1787	1.000000	86.5
accD-rpl23_p2	accD-rpl23	2	AGTATGGGATCGGTAGTGGG	ACGATACAGACGCTTGTGAC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.032581	4540	5416	1804-1860	1.000000	86.5
accD-rpl23_p3	accD-rpl23	3	TTCTCGTATGTGCTTCTGGC	TGACGATACAGACGCTTGTG	20	20	50.0	50.0	58.0	58.0	0.0	2.9	0.032957	4540	5416	1733-1789	1.000000	86.5
accD-rpl23_p4	accD-rpl23	4	AGTATGGGATCGGTAGTGGG	TGACGATACAGACGCTTGTG	20	20	55.0	50.0	58.0	58.0	0.0	2.9	0.033934	4540	5416	1806-1862	1.000000	86.5
accD-rpl23_p5	accD-rpl23	5	GTTCATGGGAGGCAGTATGG	ACGATACAGACGCTTGTGAC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.048586	4540	5416	1817-1873	1.000000	86.4
rps12-clpP_p1	rps12-clpP	1	TTTCTTATCGGCTGTCTCGC	CGTGTAGAAGGAAAGCTCCC	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.088970	27968	28740	1071-1716	1.000000	89.9
rps12-clpP_p2	rps12-clpP	2	GCATACTCCCTTCCGTTGAG	CCGTGTAGAAGGAAAGCTCC	20	20	55.0	55.0	58.1	58.0	0.0	0.0	0.070215	27968	28740	1124-1769	1.000000	89.8
rps12-clpP_p3	rps12-clpP	3	GCATACTCCCTTCCGTTGAG	CCTCCGTGTAGAAGGAAAGC	20	20	55.0	55.0	58.1	58.0	0.0	0.0	0.070215	27968	28740	1127-1772	1.000000	89.8
rps12-clpP_p4	rps12-clpP	4	GCATACTCCCTTCCGTTGAG	CGTGTAGAAGGAAAGCTCCC	20	20	55.0	55.0	58.1	58.0	0.0	0.0	0.070215	27968	28740	1123-1768	1.000000	89.8
rps12-clpP_p5	rps12-clpP	5	GCATACTCCCTTCCGTTGAG	GTCCGCAATAGGCCCATAAA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.083015	27968	28740	1256-1901	1.000000	89.3
clpP-trnL-UAG_p1	clpP-trnL-UAG	1	ACCAAACTGCGTCATACTCC	TACCAATTTCACCATGGCGG	20	20	50.0	50.0	57.9	58.5	0.0	13.0	0.625777	29686	29818	169-181	1.000000	84.3
clpP-trnL-UAG_p2	clpP-trnL-UAG	2	ACCAAACTGCGTCATACTCC	GATTCGAACCGAGATGCTCT	20	20	50.0	50.0	57.9	57.5	0.0	0.0	0.595271	29686	29818	216-228	1.000000	83.5
clpP-trnL-UAG_p3	clpP-trnL-UAG	3	ACCAAACTGCGTCATACTCC	GGATTCGAACCGAGATGCTC	20	20	50.0	55.0	57.9	58.5	0.0	0.0	0.602042	29686	29818	217-229	1.000000	83.4
clpP-trnL-UAG_p4	clpP-trnL-UAG	4	GGTTTCGGAGCAGAAGGATT	GATTCGAACCGAGATGCTCT	20	20	50.0	50.0	57.9	57.5	0.0	0.0	0.615851	29686	29818	273-285	1.000000	82.5
clpP-trnL-UAG_p5	clpP-trnL-UAG	5	GGTTTCGGAGCAGAAGGATT	GGATTCGAACCGAGATGCTC	20	20	50.0	55.0	57.9	58.5	0.0	0.0	0.622623	29686	29818	274-286	1.000000	82.4
ycf1_p1	ycf1	1	CAGGGGTTGGAAAGGACAAA	TGCTGGACATAACGAAGCTC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.090799	31067	31186	847-1183	1.000000	91.3
ycf1_p2	ycf1	2	CAGGGGTTGGAAAGGACAAA	AGTTCGTCCCTTCCTTCTGA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.091776	31067	31186	783-1119	1.000000	91.3
ycf1_p3	ycf1	3	AAACAGGGGTTGGAAAGGAC	TGCTGGACATAACGAAGCTC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.092285	31067	31186	850-1186	1.000000	91.3
ycf1_p4	ycf1	4	AAACAGGGGTTGGAAAGGAC	AGTTCGTCCCTTCCTTCTGA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.093262	31067	31186	786-1122	1.000000	91.3
ycf1_p5	ycf1	5	TGGTGGTTAGGTGCAATCAC	TGCTGGACATAACGAAGCTC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.105858	31067	31186	1392-1728	1.000000	89.0
ycf1-trnL-CAA_p1	ycf1-trnL-CAA	1	CCTGAACCCACGGTATGATG	TACCATTCCACCATCAAGGC	20	20	55.0	50.0	58.0	57.6	0.0	0.0	0.481813	38327	39436	546-1173	1.000000	88.1
ycf1-trnL-CAA_p2	ycf1-trnL-CAA	2	CCTGAACCCACGGTATGATG	CGTGTCTACCATTCCACCAT	20	20	55.0	50.0	58.0	57.3	0.0	0.0	0.737850	38327	39436	552-1179	1.000000	86.1
ycf1-trnL-CAA_p3	ycf1-trnL-CAA	3	CCTGAACCCACGGTATGATG	GCGTGTCTACCATTCCACC	20	19	55.0	57.9	58.0	58.2	0.0	0.0	1.282790	38327	39436	553-1180	1.000000	81.7
ycf1-trnL-CAA_p4	ycf1-trnL-CAA	4	GATCCCTGAACCCACGGTAT	TACCATTCCACCATCAAGGC	20	20	55.0	50.0	58.9	57.6	0.0	0.0	1.306315	38327	39436	550-1177	1.000000	81.5
ycf1-trnL-CAA_p5	ycf1-trnL-CAA	5	CCTGAACCCACGGTATGATG	ACCATTCCACCATCAAGGC	20	19	55.0	52.6	58.0	57.7	0.0	0.0	1.334540	38327	39436	545-1172	1.000000	81.3
trnL-CAA-ycf2_p1	trnL-CAA-ycf2	1	CACGCGACACTCAAAATGTC	CGATTTCTTTGGGATCCCGT	20	20	50.0	50.0	58.1	58.0	15.9	26.0	0.101027	39518	39799	901-1123	1.000000	91.2
trnL-CAA-ycf2_p2	trnL-CAA-ycf2	2	GACACGCGACACTCAAAATG	CGATTTCTTTGGGATCCCGT	20	20	50.0	50.0	58.1	58.0	15.9	26.0	0.101027	39518	39799	903-1125	1.000000	91.2
trnL-CAA-ycf2_p3	trnL-CAA-ycf2	3	AGACACGCGACACTCAAAAT	CGATTTCTTTGGGATCCCGT	20	20	45.0	50.0	58.1	58.0	15.9	26.0	0.172522	39518	39799	904-1126	1.000000	90.6
trnL-CAA-ycf2_p4	trnL-CAA-ycf2	4	ATATGTGTCCTACCTGCCGA	CGATTTCTTTGGGATCCCGT	20	20	50.0	50.0	57.9	58.0	0.0	26.0	0.120527	39518	39799	1207-1622	0.777778	79.9
trnL-CAA-ycf2_p5	trnL-CAA-ycf2	5	TACCTGCCGAATCTCCTCAA	CGATTTCTTTGGGATCCCGT	20	20	50.0	50.0	58.1	58.0	0.0	26.0	0.177746	39518	39799	1197-1612	0.777778	79.5
rps12-trnV-GAC_p1	rps12-trnV-GAC	1	ATCGTACTCTGGCGACTTTG	GACTTTCACCACGTCAAGGT	20	20	50.0	50.0	57.7	58.1	0.0	0.0	0.330117	51533	52515	972-1265	1.000000	84.4
rps12-trnV-GAC_p2	rps12-trnV-GAC	2	ATCGTACTCTGGCGACTTTG	TGACTTTCACCACGTCAAGG	20	20	50.0	50.0	57.7	58.1	0.0	0.0	0.331478	51533	52515	973-1266	1.000000	84.4
rps12-trnV-GAC_p3	rps12-trnV-GAC	3	GCGTAAGGCAGAGTTTGGTT	GACTTTCACCACGTCAAGGT	20	20	50.0	50.0	58.8	58.1	0.0	0.0	0.814811	51533	52515	953-1246	1.000000	80.6
rps12-trnV-GAC_p4	rps12-trnV-GAC	4	GCGTAAGGCAGAGTTTGGTT	TGACTTTCACCACGTCAAGG	20	20	50.0	50.0	58.8	58.1	0.0	0.0	0.816172	51533	52515	954-1247	1.000000	80.6
rps12-trnV-GAC_p5	rps12-trnV-GAC	5	ATCGTACTCTGGCGACTTTG	AGGTGACACTCTACCACTGA	20	20	50.0	50.0	57.7	57.3	0.0	5.4	0.957125	51533	52515	956-1249	1.000000	79.4
trnV-GAC-rrn16_p1	trnV-GAC-rrn16	1	ACCTTGACGTGGTGAAAGTC	GGGCAGGTTCTTACGCATTA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.083215	52588	53143	719-810	1.000000	84.2
trnV-GAC-rrn16_p2	trnV-GAC-rrn16	2	CCTTGACGTGGTGAAAGTCA	GGGCAGGTTCTTACGCATTA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.084576	52588	53143	718-809	1.000000	84.2
trnV-GAC-rrn16_p3	trnV-GAC-rrn16	3	ACCTTGACGTGGTGAAAGTC	TACGCATTACTCACCCGTTC	20	20	50.0	50.0	58.1	57.7	0.0	0.0	0.333235	52588	53143	708-799	1.000000	82.3
trnV-GAC-rrn16_p4	trnV-GAC-rrn16	4	CCTTGACGTGGTGAAAGTCA	TACGCATTACTCACCCGTTC	20	20	50.0	50.0	58.1	57.7	0.0	0.0	0.334596	52588	53143	707-798	1.000000	82.3
trnV-GAC-rrn16_p5	trnV-GAC-rrn16	5	ACCTTGACGTGGTGAAAGTC	CTTACGCATTACTCACCCGT	20	20	50.0	50.0	58.1	57.7	0.0	0.0	0.339495	52588	53143	710-801	1.000000	82.2
rrn16-trnI-GAU_p1	rrn16-trnI-GAU	1	AGTGACTGGAGTGAAGTCGT	CTACCACTGAGCTAATAGCCC	20	21	50.0	52.4	58.3	57.3	0.0	8.9	1.975625	54628	55682	821-1127	1.000000	73.0
rrn16-trnI-GAU_p2	rrn16-trnI-GAU	2	AAGTCGTAACAAGGTAGCCG	CTACCACTGAGCTAATAGCCC	20	21	50.0	52.4	57.6	57.3	0.0	8.9	2.028190	54628	55682	808-1114	1.000000	72.6
rrn16-trnI-GAU_p3	rrn16-trnI-GAU	3	AGTGACTGGAGTGAAGTCGT	TACCACTGAGCTAATAGCCC	20	20	50.0	50.0	58.3	56.1	0.0	8.9	2.195047	54628	55682	820-1126	1.000000	71.2
rrn16-trnI-GAU_p4	rrn16-trnI-GAU	4	AAGTCGTAACAAGGTAGCCG	TACCACTGAGCTAATAGCCC	20	20	50.0	50.0	57.6	56.1	0.0	8.9	2.247612	54628	55682	807-1113	1.000000	70.9
rrn16-trnI-GAU_p5	rrn16-trnI-GAU	5	GGCTAGTGACTGGAGTGAAG	CTACCACTGAGCTAATAGCCC	20	21	55.0	52.4	57.3	57.3	0.0	8.9	2.333078	54628	55682	825-1131	1.000000	70.1
rrn5-trnR-ACG_p1	rrn5-trnR-ACG	1	AATCCATCCCGAACTTGGTG	CCACGTGCTCTAATCCTCTG	20	20	50.0	55.0	57.9	57.8	0.0	10.9	0.344678	61171	61284	224-248	1.000000	82.7
rrn5-trnR-ACG_p2	rrn5-trnR-ACG	2	ACCAATCCATCCCGAACTTG	CCACGTGCTCTAATCCTCTG	20	20	50.0	55.0	57.9	57.8	0.0	10.9	0.344678	61171	61284	227-251	1.000000	82.7
rrn5-trnR-ACG_p3	rrn5-trnR-ACG	3	CAATCCATCCCGAACTTGGT	CCACGTGCTCTAATCCTCTG	20	20	50.0	55.0	57.9	57.8	0.0	10.9	0.344678	61171	61284	225-249	1.000000	82.7
rrn5-trnR-ACG_p4	rrn5-trnR-ACG	4	CACCAATCCATCCCGAACTT	CCACGTGCTCTAATCCTCTG	20	20	50.0	55.0	57.9	57.8	0.0	10.9	0.344678	61171	61284	228-252	1.000000	82.6
rrn5-trnR-ACG_p5	rrn5-trnR-ACG	5	GGGAAGCCCTGTGGAAAAAT	CCACGTGCTCTAATCCTCTG	20	20	50.0	55.0	58.4	57.8	0.0	10.9	0.571398	61171	61284	173-197	1.000000	82.4
ycf3-psaA_p1	ycf3-psaA	1	AGCTGCTGAATATTGGAAGCA	TGGGTCCCTTAGCTAGTGTC	21	20	42.9	55.0	58.0	58.1	16.1	10.0	1.146854	78999	79538	754-765	1.000000	72.7
ycf3-psaA_p2	ycf3-psaA	2	AGCTGCTGAATATTGGAAGCA	GGGTCCCTTAGCTAGTGTCT	21	20	42.9	55.0	58.0	57.8	16.1	0.0	1.171100	78999	79538	753-764	1.000000	72.5
ycf3-psaA_p3	ycf3-psaA	3	AGCTGCTGAATATTGGAAGCA	TTGGGTCCCTTAGCTAGTGT	21	20	42.9	50.0	58.0	57.7	16.1	10.0	1.346194	78999	79538	755-766	1.000000	71.1
ycf3-psaA_p4	ycf3-psaA	4	TGGAAGCAAGCTATAGCACT	TGGGTCCCTTAGCTAGTGTC	20	20	45.0	55.0	56.6	58.1	26.1	10.0	1.519012	78999	79538	741-752	1.000000	69.8
ycf3-psaA_p5	ycf3-psaA	5	TGGAAGCAAGCTATAGCACT	GGGTCCCTTAGCTAGTGTCT	20	20	45.0	55.0	56.6	57.8	26.1	0.0	1.543259	78999	79538	740-751	1.000000	69.6
psbM-petN_p1	psbM-petN	1	GCACAAACGAAGCCTCAAAG	TGGGCTGCTCTAATGGTAGT	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.361642	90354	91045	751-794	1.000000	84.6
psbM-petN_p2	psbM-petN	2	ATTTACGCACAAACGAAGCC	TGGGCTGCTCTAATGGTAGT	20	20	45.0	50.0	57.7	58.1	0.0	0.0	0.445935	90354	91045	757-800	1.000000	83.9
psbM-petN_p3	psbM-petN	3	TATTCATTGCAGTGCCCACT	TGGGCTGCTCTAATGGTAGT	20	20	45.0	50.0	57.5	58.1	13.7	0.0	0.633117	90354	91045	792-835	1.000000	82.2
psbM-petN_p4	psbM-petN	4	CGTCATTTACGCACAAACGA	TGGGCTGCTCTAATGGTAGT	20	20	45.0	50.0	57.4	58.1	0.0	0.0	0.684095	90354	91045	761-804	1.000000	81.9
psbM-petN_p5	psbM-petN	5	ACGAAGCCTCAAAGTGAACT	TGGGCTGCTCTAATGGTAGT	20	20	45.0	50.0	57.4	58.1	0.0	0.0	0.742489	90354	91045	745-788	1.000000	81.5
atpF-atpA_p1	atpF-atpA	1	TATCGACTTGCTCCTAGCCA	ACTGATTTCATCGGGTCGAA	20	20	50.0	45.0	57.9	56.9	0.0	0.0	1.118275	106019	106182	228-285	1.000000	82.3
atpF-atpA_p2	atpF-atpA	2	TATCGACTTGCTCCTAGCCA	AGCAATGCCATCTCCTACTTG	20	21	50.0	47.6	57.9	58.1	0.0	0.0	1.139032	106019	106182	318-375	1.000000	80.2
atpF-atpA_p3	atpF-atpA	3	TATCGACTTGCTCCTAGCCA	GCAATGCCATCTCCTACTTGA	20	21	50.0	47.6	57.9	58.1	0.0	0.0	1.144245	106019	106182	317-374	1.000000	80.1
atpF-atpA_p4	atpF-atpA	4	TATCGACTTGCTCCTAGCCA	AGCAATGCCATCTCCTACTT	20	20	50.0	45.0	57.9	56.6	0.0	0.0	1.481290	106019	106182	318-375	1.000000	77.4
atpF-atpA_p5	atpF-atpA	5	TATCGACTTGCTCCTAGCCA	GCAATGCCATCTCCTACTTG	20	20	50.0	50.0	57.9	56.6	0.0	0.0	1.504496	106019	106182	317-374	1.000000	77.3
trnK-UUU_p1	trnK-UUU	1	AACTGCTCTACTTCGACTGT	GGATGCTACCTATCTTGCTTGT	20	22	45.0	45.5	56.2	57.9	0.0	0.0	3.846460	113833	116328	2528-2633	1.000000	55.4
trnK-UUU_p2	trnK-UUU	2	AACTGCTCTACTTCGACTGT	AGGATGCTACCTATCTTGCTTG	20	22	45.0	45.5	56.2	57.7	0.0	0.6	4.116684	113833	116328	2529-2634	1.000000	53.2
trnK-UUU_p3	trnK-UUU	3	AACTGCTCTACTTCGACTGT	AGGATGCTACCTATCTTGCT	20	20	45.0	45.0	56.2	55.4	0.0	0.6	4.382363	113833	116328	2529-2634	1.000000	51.1
trnK-UUU_p4	trnK-UUU	4	AACTGCTCTACTTCGACTGT	AGGTGCTAGATTCTGGTACTC	20	21	45.0	47.6	56.2	56.3	0.0	15.7	4.508658	113833	116328	2441-2551	1.000000	50.2
trnK-UUU_p5	trnK-UUU	5	GTAACTGCTCTACTTCGACTGT	GGATGCTACCTATCTTGCTTGT	22	22	45.5	45.5	57.6	57.9	0.0	0.0	4.501185	113833	116328	2530-2635	1.000000	50.1
atpB-atpE_p1	atpB-atpE	1	GAAGCTACCGCAAAGGCTAT	ACAACTCGATTGGGAGTGAG	20	20	50.0	50.0	58.0	57.2	0.0	10.8	0.796239	126744	126753	88-93	1.000000	78.1
atpB-atpE_p2	atpB-atpE	2	GAAGCTACCGCAAAGGCTAT	CAACTCGATTGGGAGTGAGT	20	20	50.0	50.0	58.0	57.2	0.0	0.0	0.796239	126744	126753	87-92	1.000000	78.1
atpB-atpE_p3	atpB-atpE	3	ACCGCAAAGGCTATGAACTT	CAACTCGATTGGGAGTGAGT	20	20	45.0	50.0	57.8	57.2	0.0	0.0	0.951539	126744	126753	81-86	1.000000	77.2
atpB-atpE_p4	atpB-atpE	4	TGGTCTACCCGAACAGTCTT	ACAACTCGATTGGGAGTGAG	20	20	50.0	50.0	58.0	57.2	0.0	10.8	0.756537	126744	126753	131-136	1.000000	77.0
atpB-atpE_p5	atpB-atpE	5	TGGTCTACCCGAACAGTCTT	CAACTCGATTGGGAGTGAGT	20	20	50.0	50.0	58.0	57.2	0.0	0.0	0.756537	126744	126753	130-135	1.000000	77.0