Hotspot

atpF

LSC · 13287-14596 · 1310 bp

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Pi 0.00165

MarkerSeek score 28.70

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1310
variable_sites: 7
pi: 0.00165
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 28.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	CACCCGCCATTACTTCATCA	ATGGGGAAGGACTGATTGGA	50.0	50.0	58.0	58.0	1903-1936 bp	1.0	80.9
atpF_p2	CACCCGCCATTACTTCATCA	GGACTGGTTGTAGCCTTAGC	50.0	55.0	58.0	58.0	2066-2100 bp	1.0	80.8
atpF_p3	CACCCGCCATTACTTCATCA	ATTTCTGCCGCTTCCGTTAT	50.0	45.0	58.0	58.0	2252-2286 bp	1.0	80.6
atpF_p4	CACCCGCCATTACTTCATCA	GCCAAGCTGTAGAAGGGATC	50.0	55.0	58.0	58.0	2167-2201 bp	1.0	80.6
atpF_p5	CCATTGCTCCCCTTTCTTGT	ATGGGGAAGGACTGATTGGA	50.0	50.0	58.1	58.0	2407-2440 bp	1.0	80.4