Hotspot

rpl32-trnL-UAG

SSC · 119266-119992 · 727 bp

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Pi 0.00715

MarkerSeek score 56.20

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 727
variable_sites: 17
pi: 0.00715
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AGGAAGGGGTATTGGGTAGC	AGCGTGTCTACCGATTTCAC	55.0	50.0	58.5	58.0	832-858 bp	1.0	80.5
rpl32-trnL-UAG_p2	AGGAAGGGGTATTGGGTAGC	CAGCGTGTCTACCGATTTCA	55.0	50.0	58.5	58.0	833-859 bp	1.0	80.5
rpl32-trnL-UAG_p3	AGGAAGGGGTATTGGGTAGC	TCCTAAGAGCAGCGTGTCTA	55.0	50.0	58.5	57.9	842-868 bp	1.0	79.5
rpl32-trnL-UAG_p4	AGGAAGGGGTATTGGGTAGC	CCTAAGAGCAGCGTGTCTAC	55.0	55.0	58.5	57.8	841-867 bp	1.0	78.9
rpl32-trnL-UAG_p5	AGGAAGGGGTATTGGGTAGC	CTAAGAGCAGCGTGTCTACC	55.0	55.0	58.5	57.8	840-866 bp	1.0	78.9