Hotspot

ycf3-trnS-GGA

LSC · 49072-49994 · 923 bp

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Pi 0.00368

MarkerSeek score 52.10

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 923
variable_sites: 12
pi: 0.00368
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	CCATTTATCCGAGATCTGGGC	CCCTCGGTAAACAAAAGCCT	52.4	50.0	58.3	58.1	1011-1023 bp	1.0	70.3
ycf3-trnS-GGA_p2	CCATTTATCCGAGATCTGGGC	ACGGAAAGAGAGGGATTCGA	52.4	50.0	58.3	58.2	1032-1044 bp	1.0	69.8
ycf3-trnS-GGA_p3	CCATTTATCCGAGATCTGGGC	TTCCAATGCTACGCCTTGAA	52.4	45.0	58.3	57.8	982-994 bp	1.0	69.6
ycf3-trnS-GGA_p4	CATTTATCCGAGATCTGGGCA	CCCTCGGTAAACAAAAGCCT	47.6	50.0	57.6	58.1	1010-1022 bp	1.0	69.4
ycf3-trnS-GGA_p5	CATTTATCCGAGATCTGGGCA	ACGGAAAGAGAGGGATTCGA	47.6	50.0	57.6	58.2	1031-1043 bp	1.0	68.9