Hotspot

trnM-CAU-psbD

LSC · 35479-37094 · 1616 bp

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Pi 0.00858

MarkerSeek score 67.70

Primer available yes

Label trnM-CAU-psbD

Feature Metadata

label_name: trnM-CAU-psbD
feature_type: igs
region: LSC
length_bp: 1616
variable_sites: 35
pi: 0.00858
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-psbD_p1	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	1684-1759 bp	1.0	83.1
trnM-CAU-psbD_p2	GTGGTAGAGTAACGCCATGG	GACCGGACCAACCTACAAAA	55.0	50.0	58.1	57.7	1698-1773 bp	1.0	83.0
trnM-CAU-psbD_p3	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.1	58.2	1687-1762 bp	1.0	83.0
trnM-CAU-psbD_p4	TCAGTGGTAGAGTAACGCCA	GACCGGACCAACCTACAAAA	50.0	50.0	58.1	57.7	1701-1776 bp	1.0	82.8
trnM-CAU-psbD_p5	GTGGTAGAGTAACGCCATGG	TAGACCGGACCAACCTACAA	55.0	50.0	58.1	57.7	1700-1775 bp	1.0	82.6