Hotspot

atpA-atpF

LSC · 13222-13286 · 65 bp

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Pi 0.0806

MarkerSeek score 63.10

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 65
variable_sites: 12
pi: 0.0806
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	CATCTGCTCGAGTGGTTACC	TCATGTTTGGTTCGGGAAGG	55.0	50.0	58.1	58.1	562-593 bp	1.0	81.1
atpA-atpF_p2	CACCCGCCATTACTTCATCA	GGGCTATTGAACAGCTGGAA	50.0	50.0	58.0	57.9	557-588 bp	1.0	81.0
atpA-atpF_p3	CACCCGCCATTACTTCATCA	TCATGTTTGGTTCGGGAAGG	50.0	50.0	58.0	58.1	694-725 bp	1.0	80.9
atpA-atpF_p4	GTCGCCTACTTGAAGTACGG	TCATGTTTGGTTCGGGAAGG	55.0	50.0	58.1	58.1	651-682 bp	1.0	80.6
atpA-atpF_p5	CCATTGCTCCCCTTTCTTGT	TCATGTTTGGTTCGGGAAGG	50.0	50.0	58.1	58.1	1198-1229 bp	1.0	80.0