Hotspot

trnK-UUU-rps16

LSC · 4244-5303 · 1060 bp

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Pi 0.01

MarkerSeek score 57.20

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 1060
variable_sites: 31
pi: 0.01
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	TCAGTCGCGGTCTTACAAAG	TCAAAGAAGGCGGGAGTTTT	50.0	45.0	57.9	57.6	1201-1240 bp	1.0	81.7
trnK-UUU-rps16_p2	TCAGTCGCGGTCTTACAAAG	TTCAAAGAAGGCGGGAGTTT	50.0	45.0	57.9	57.6	1202-1241 bp	1.0	81.7
trnK-UUU-rps16_p3	TCAGTCGCGGTCTTACAAAG	TTTCAAAGAAGGCGGGAGTT	50.0	45.0	57.9	57.6	1203-1242 bp	1.0	81.7
trnK-UUU-rps16_p4	AAGCCGAGTACTCTACCGTT	TCAAAGAAGGCGGGAGTTTT	50.0	45.0	58.2	57.6	1114-1153 bp	1.0	81.1
trnK-UUU-rps16_p5	AAGCCGAGTACTCTACCGTT	TTCAAAGAAGGCGGGAGTTT	50.0	45.0	58.2	57.6	1115-1154 bp	1.0	81.1