Hotspot

ycf3

Genome · 46785-48652 · 1868 bp

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Pi 0.00863
MarkerSeek score 50.70
Primer available yes
Label ycf3

Feature Metadata

label_name
ycf3
feature_type
gene
region
Genome
length_bp
1868
variable_sites
16
pi
0.00863
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
50.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3_p1 CTCCTCTTTTCAGACGAGGC ATGGCTCCAACAATTCCCAG 55.0 50.0 58.0 58.1 2878-2886 bp 1.0 80.0
ycf3_p2 CTCCTCTTTTCAGACGAGGC ATTCGAACCCTCGGTAGACA 55.0 50.0 58.0 58.2 2489-2497 bp 1.0 80.0
ycf3_p3 TTTGACTTCTGGTTCCGGTG ATGGCTCCAACAATTCCCAG 50.0 50.0 58.0 58.1 2918-2926 bp 1.0 79.7
ycf3_p4 TTTGACTTCTGGTTCCGGTG ATTCGAACCCTCGGTAGACA 50.0 50.0 58.0 58.2 2529-2537 bp 1.0 79.7
ycf3_p5 GTTATTCCAGATGCTCGCCA ATTCGAACCCTCGGTAGACA 50.0 50.0 58.0 58.2 2948-2956 bp 1.0 79.5