Hotspot

ycf2

Genome · 30544-36675 · 6132 bp

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Pi 0.00962
MarkerSeek score 43.60
Primer available yes
Label ycf2

Feature Metadata

label_name
ycf2
feature_type
gene
region
Genome
length_bp
6132
variable_sites
59
pi
0.00962
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
43.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf2_p1 TGATTTCAAGCAGGTCAAAGA AGCTGTTTTCCAAACGATTT 38.1 35.0 55.7 54.3 1115-1118 bp 1.0 41.0
ycf2_p2 TGATTTCAAGCAGGTCAAAGA ATCGGTTCCATTCTTTCTGA 38.1 40.0 55.7 54.4 803 bp 1.0 40.9
ycf2_p3 TGATTTCAAGCAGGTCAAAGA TCGGTTCCATTCTTTCTGAT 38.1 40.0 55.7 54.4 802 bp 1.0 40.9
ycf2_p4 AGATGATTTCAAGCAGGTCA ATCGGTTCCATTCTTTCTGA 40.0 40.0 54.6 54.4 806 bp 1.0 40.9
ycf2_p5 AGATGATTTCAAGCAGGTCA TCGGTTCCATTCTTTCTGAT 40.0 40.0 54.6 54.4 805 bp 1.0 40.9