Hotspot

trnG-UCC

Genome · 41353-41422 · 70 bp

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Pi 0.0429
MarkerSeek score 65.40
Primer available yes
Label trnG-UCC

Feature Metadata

label_name
trnG-UCC
feature_type
tRNA
region
Genome
length_bp
70
variable_sites
3
pi
0.0429
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC_p1 CGGCCATCTCTCCAAAATGA GGGATAGTGCTCCTAGTCGT 50.0 55.0 58.0 58.0 1020-1029 bp 1.0 80.3
trnG-UCC_p2 TCGGCCATCTCTCCAAAATG GGGATAGTGCTCCTAGTCGT 50.0 55.0 58.0 58.0 1021-1030 bp 1.0 80.3
trnG-UCC_p3 CGGCCATCTCTCCAAAATGA GCTCACGAACGTACTCCTTT 50.0 50.0 58.0 57.9 1375-1384 bp 1.0 80.0
trnG-UCC_p4 TCGGCCATCTCTCCAAAATG GCTCACGAACGTACTCCTTT 50.0 50.0 58.0 57.9 1376-1385 bp 1.0 80.0
trnG-UCC_p5 CGGCCATCTCTCCAAAATGA GTTCGAATCCTATCTCCGCC 50.0 55.0 58.0 57.9 710-719 bp 1.0 79.8