Hotspot

rpoC1

Genome · 9255-11907 · 2653 bp

Back to Jubula

Pi 0.00943
MarkerSeek score 55.70
Primer available yes
Label rpoC1

Feature Metadata

label_name
rpoC1
feature_type
gene
region
Genome
length_bp
2653
variable_sites
25
pi
0.00943
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoC1_p1 ACTTGTTCGAGAATTACGATCT ACAAACATACATCAACAATGAGT 36.4 30.4 55.3 54.8 2864 bp 1.0 40.8
rpoC1_p2 TGTTCGAGAATTACGATCTTTAGC ACAAACATACATCAACAATGAGT 37.5 30.4 57.2 54.8 2861 bp 1.0 40.8
rpoC1_p3 ACTTGTTCGAGAATTACGATCT ACAAACATACATCAACAATGAGTT 36.4 29.2 55.3 55.5 2864 bp 1.0 40.8
rpoC1_p4 ACTTGTTCGAGAATTACGATCTT ACAAACATACATCAACAATGAGT 34.8 30.4 56.1 54.8 2864 bp 1.0 40.8
rpoC1_p5 GTTCGAGAATTACGATCTTTAGCT ACAAACATACATCAACAATGAGT 37.5 30.4 56.9 54.8 2860 bp 1.0 40.8