Hotspot

rps15-ycf1

Genome · 101205-102812 · 1608 bp

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Pi 0.0112
MarkerSeek score 58.70
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
Genome
length_bp
1608
variable_sites
18
pi
0.0112
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 AGAAATGGAGGAAAGAGAGGT AGATCAATTATAGGATAAATATGGAGA 42.9 25.9 56.0 53.7 1712 bp 1.0 40.8
rps15-ycf1_p2 AGAAATGGAGGAAAGAGAGGT GATCAATTATAGGATAAATATGGAGA 42.9 26.9 56.0 52.4 1711 bp 1.0 40.8
rps15-ycf1_p3 AGAAATGGAGGAAAGAGAGGT AGATCAATTATAGGATAAATATGGAG 42.9 26.9 56.0 52.4 1712 bp 1.0 40.8
rps15-ycf1_p4 TGGAGGAAAGAGAGGTAAAGT AGATCAATTATAGGATAAATATGGAGA 42.9 25.9 55.6 53.7 1707 bp 1.0 40.8
rps15-ycf1_p5 TGGAGGAAAGAGAGGTAAAGTT AGATCAATTATAGGATAAATATGGAGA 40.9 25.9 56.4 53.7 1707 bp 1.0 40.8