Hotspot

ndhH-rps15

Genome · 100894-100937 · 44 bp

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Pi 0.0455
MarkerSeek score 64.20
Primer available yes
Label ndhH-rps15

Feature Metadata

label_name
ndhH-rps15
feature_type
igs
region
Genome
length_bp
44
variable_sites
2
pi
0.0455
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhH-rps15_p1 AGTCGTAAAACACCGTGCAT ATTTTGAGCCAACGTAAGCG 45.0 45.0 58.1 57.4 235 bp 1.0 75.1
ndhH-rps15_p2 AACACCGTGCATTGATGGAT ATTTTGAGCCAACGTAAGCG 45.0 45.0 58.2 57.4 227 bp 1.0 74.9
ndhH-rps15_p3 TGATGGATGATGAGGACCCA ATTTTGAGCCAACGTAAGCG 50.0 45.0 57.8 57.4 215 bp 1.0 74.7
ndhH-rps15_p4 ATTCGCATACCAGTAGCAGC ATTTTGAGCCAACGTAAGCG 50.0 45.0 58.1 57.4 610 bp 1.0 74.6
ndhH-rps15_p5 GGATTCCTGATGCTCGTAGC ATTTTGAGCCAACGTAAGCG 55.0 45.0 58.2 57.4 840 bp 1.0 73.8