Hotspot

ccsA-ndhD

Genome · 94066-94213 · 148 bp

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Pi 0.0338
MarkerSeek score 66.00
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
Genome
length_bp
148
variable_sites
5
pi
0.0338
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 TGGCGAACAAAACAATCAGC TCGTTTTATCTGTCTGGGATTCT 45.0 39.1 57.9 57.3 333-336 bp 1.0 50.7
ccsA-ndhD_p2 TGGCGAACAAAACAATCAGC TGTCTGGGATTCTAAAACAGAGT 45.0 39.1 57.9 57.2 323-326 bp 1.0 49.6
ccsA-ndhD_p3 CGAACAAAACAATCAGCCGT TCGTTTTATCTGTCTGGGATTCT 45.0 39.1 57.6 57.3 330-333 bp 1.0 48.6
ccsA-ndhD_p4 TGGCGAACAAAACAATCAGC TCTGTCTGGGATTCTAAAACAGAG 45.0 41.7 57.9 58.0 325-328 bp 1.0 47.8
ccsA-ndhD_p5 TGGCGAACAAAACAATCAGC TCTGTCTGGGATTCTAAAACAGA 45.0 39.1 57.9 56.9 325-328 bp 1.0 47.5