Hotspot

trnR-ACG-trnN-GUU

Genome · 88186-88786 · 601 bp

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Pi 0.0207
MarkerSeek score 67.40
Primer available yes
Label trnR-ACG-trnN-GUU

Feature Metadata

label_name
trnR-ACG-trnN-GUU
feature_type
igs
region
Genome
length_bp
601
variable_sites
12
pi
0.0207
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-ACG-trnN-GUU_p1 TACAGATCAAGTAGGGGCGG TTCCTCAGTAGCTCAGTGGT 55.0 50.0 58.6 57.7 767-1084 bp 1.0 75.6
trnR-ACG-trnN-GUU_p2 TACAGATCAAGTAGGGGCGG GGCTGTTAACCGATTTGTCG 55.0 50.0 58.6 57.5 738-1055 bp 1.0 74.1
trnR-ACG-trnN-GUU_p3 CTCAGTGGAGTAGAGCACGT TTCCTCAGTAGCTCAGTGGT 55.0 50.0 58.8 57.7 738-1055 bp 1.0 73.8
trnR-ACG-trnN-GUU_p4 CTCAGTGGAGTAGAGCACGT GGCTGTTAACCGATTTGTCG 55.0 50.0 58.8 57.5 709-1026 bp 1.0 72.2
trnR-ACG-trnN-GUU_p5 TCAGTGGAGTAGAGCACGTG TTCCTCAGTAGCTCAGTGGT 55.0 50.0 59.1 57.7 737-1054 bp 1.0 71.5