Hotspot

rrn5-trnR-ACG

Genome · 87972-88111 · 140 bp

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Pi 0.0299
MarkerSeek score 69.80
Primer available yes
Label rrn5-trnR-ACG

Feature Metadata

label_name
rrn5-trnR-ACG
feature_type
igs
region
Genome
length_bp
140
variable_sites
4
pi
0.0299
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rrn5-trnR-ACG_p1 CCCACGGAAAAATAGCTCGA TGAAACTCTGAGGCTGGTTG 50.0 50.0 58.0 57.7 257-263 bp 1.0 80.8
rrn5-trnR-ACG_p2 CCCACGGAAAAATAGCTCGA TGTTGAAACTCTGAGGCTGG 50.0 50.0 58.0 57.7 260-266 bp 1.0 80.8
rrn5-trnR-ACG_p3 CCCACGGAAAAATAGCTCGA GTTGAAACTCTGAGGCTGGT 50.0 50.0 58.0 57.7 259-265 bp 1.0 80.8
rrn5-trnR-ACG_p4 AATCCATCCCGAACTTGGTG TGAAACTCTGAGGCTGGTTG 50.0 50.0 57.9 57.7 315-321 bp 1.0 79.4
rrn5-trnR-ACG_p5 AATCCATCCCGAACTTGGTG TGTTGAAACTCTGAGGCTGG 50.0 50.0 57.9 57.7 318-324 bp 1.0 79.4