Hotspot

trnT-UGU-trnL-UAA

Genome · 49881-50099 · 219 bp

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Pi 0.0229
MarkerSeek score 66.00
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
Genome
length_bp
219
variable_sites
5
pi
0.0229
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GAGTCGAACCGATGACCATC AAATTAAGTCCGCAGCGTCT 55.0 45.0 58.2 57.9 320-322 bp 1.0 79.5
trnT-UGU-trnL-UAA_p2 GAGTCGAACCGATGACCATC ATTAAGTCCGCAGCGTCTAC 55.0 50.0 58.2 57.8 318-320 bp 1.0 78.6
trnT-UGU-trnL-UAA_p3 GAGTCGAACCGATGACCATC TACCAATTTCGCCATACCCC 55.0 50.0 58.2 57.6 301-303 bp 1.0 77.5
trnT-UGU-trnL-UAA_p4 GACCATCGCATTACAAGTGC AAATTAAGTCCGCAGCGTCT 50.0 45.0 57.5 57.9 307-309 bp 1.0 77.1
trnT-UGU-trnL-UAA_p5 GACCATCGCATTACAAGTGC ATTAAGTCCGCAGCGTCTAC 50.0 50.0 57.5 57.8 305-307 bp 1.0 76.3