Hotspot

chlB-trnK

Genome · 25941-26391 · 451 bp

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Pi 0.0222
MarkerSeek score 55.90
Primer available yes
Label chlB-trnK

Feature Metadata

label_name
chlB-trnK
feature_type
igs
region
Genome
length_bp
451
variable_sites
10
pi
0.0222
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
chlB-trnK_p1 AAGCTGCTTGGTTTTCGAGA CAAGTCGCACTTAAAAGCCG 45.0 50.0 58.0 58.0 1172-1173 bp 1.0 80.1
chlB-trnK_p2 CAAGCTGCTTGGTTTTCGAG CAAGTCGCACTTAAAAGCCG 50.0 50.0 58.0 58.0 1173-1174 bp 1.0 80.0
chlB-trnK_p3 CTCAAGCTGCTTGGTTTTCG CAAGTCGCACTTAAAAGCCG 50.0 50.0 58.0 58.0 1175-1176 bp 1.0 80.0
chlB-trnK_p4 TGTAAGTTGTGCAGGCACTT CAAGTCGCACTTAAAAGCCG 45.0 50.0 57.9 58.0 1048-1049 bp 1.0 80.0
chlB-trnK_p5 AAGCTGTAGTTTTCGGCGAT CAAGTCGCACTTAAAAGCCG 45.0 50.0 57.9 58.0 1121-1122 bp 1.0 79.6