Hotspot

psbl-psbK

Genome · 23476-23767 · 292 bp

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Pi 0.0412
MarkerSeek score 76.50
Primer available yes
Label psbl-psbK

Feature Metadata

label_name
psbl-psbK
feature_type
igs
region
Genome
length_bp
292
variable_sites
12
pi
0.0412
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbl-psbK_p1 TCTTTACGGCCAGGATTTCG GCTTTTGTTTGGCAAGCTTC 50.0 45.0 58.0 57.0 435-448 bp 1.0 74.0
psbl-psbK_p2 CTTTACGGCCAGGATTTCGT GCTTTTGTTTGGCAAGCTTC 50.0 45.0 58.3 57.0 434-447 bp 1.0 72.0
psbl-psbK_p3 TTTACGGCCAGGATTTCGTC GCTTTTGTTTGGCAAGCTTC 50.0 45.0 58.3 57.0 433-446 bp 1.0 72.0
psbl-psbK_p4 TCTTTACGGCCAGGATTTCG TTTGTTTGGCAAGCTTCAGT 50.0 40.0 58.0 56.7 432-445 bp 1.0 71.5
psbl-psbK_p5 CTTTACGGCCAGGATTTCGT TTTGTTTGGCAAGCTTCAGT 50.0 40.0 58.3 56.7 431-444 bp 1.0 69.5