Hotspot

psaM-trnS-GCU

Genome · 22963-23250 · 288 bp

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Pi 0.0347
MarkerSeek score 72.50
Primer available yes
Label psaM-trnS-GCU

Feature Metadata

label_name
psaM-trnS-GCU
feature_type
igs
region
Genome
length_bp
288
variable_sites
10
pi
0.0347
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaM-trnS-GCU_p1 ACAATGGTCCCAATTTCGGA CGGAGAGAGAGGGATTCGAA 45.0 55.0 57.4 58.3 478 bp 1.0 74.4
psaM-trnS-GCU_p2 ACAATGGTCCCAATTTCGGA AACGGAGAGAGAGGGATTCG 45.0 55.0 57.4 58.6 480 bp 1.0 72.1
psaM-trnS-GCU_p3 ACAATGGTCCCAATTTCGGA GATTCGAACCCTCGGTACAA 45.0 50.0 57.4 57.3 466 bp 1.0 71.7
psaM-trnS-GCU_p4 ACAATGGTCCCAATTTCGGAT CGGAGAGAGAGGGATTCGAA 42.9 55.0 57.9 58.3 478 bp 1.0 70.3
psaM-trnS-GCU_p5 AACAATGGTCCCAATTTCGGA CGGAGAGAGAGGGATTCGAA 42.9 55.0 58.1 58.3 479 bp 1.0 70.2