Hotspot

atpA-trnR-UCU

Genome · 21421-21484 · 64 bp

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Pi 0.0781
MarkerSeek score 75.20
Primer available yes
Label atpA-trnR-UCU

Feature Metadata

label_name
atpA-trnR-UCU
feature_type
igs
region
Genome
length_bp
64
variable_sites
5
pi
0.0781
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpA-trnR-UCU_p1 TCGTTCCACTAAAGTTTTTACAGA GTTCGAATCCTATTGGGCGT 33.3 50.0 56.5 58.1 182 bp 1.0 42.2
atpA-trnR-UCU_p2 CGTTCCACTAAAGTTTTTACAGA GTTCGAATCCTATTGGGCGT 34.8 50.0 55.2 58.1 181 bp 1.0 42.2
atpA-trnR-UCU_p3 TCGTTCCACTAAAGTTTTTACAG GTTCGAATCCTATTGGGCGT 34.8 50.0 55.2 58.1 182 bp 1.0 42.2
atpA-trnR-UCU_p4 TCGTTCCACTAAAGTTTTTACAGAA GTTCGAATCCTATTGGGCGT 32.0 50.0 57.2 58.1 182 bp 1.0 42.2
atpA-trnR-UCU_p5 TTCGTTCCACTAAAGTTTTTACAGA GTTCGAATCCTATTGGGCGT 32.0 50.0 57.2 58.1 183 bp 1.0 42.2