Hotspot

rps2

LSC · 15428-16138 · 711 bp

Back to Jovibarba

Pi 0

MarkerSeek score 40.00

Primer available yes

Label rps2

Feature Metadata

label_name: rps2
feature_type: gene
region: LSC
length_bp: 711
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2_p1	CCAGACCCGTACAAGCAATT	TCAGGGTACGTCGTCTCTTT	50.0	50.0	58.2	58.1	835 bp	1.0	77.8
rps2_p2	CCAGACCCGTACAAGCAATT	TTCAGGGTACGTCGTCTCTT	50.0	50.0	58.2	58.1	836 bp	1.0	77.8
rps2_p3	CCAGACCCGTACAAGCAATT	TTTCAGGGTACGTCGTCTCT	50.0	50.0	58.2	58.1	837 bp	1.0	77.8
rps2_p4	CCAGACCCGTACAAGCAATT	GCCAATCTCCGGTAGAAGTC	50.0	55.0	58.2	57.8	887 bp	1.0	76.8
rps2_p5	CCAGACCCGTACAAGCAATT	CGGTAGAAGTCAAGGTTCCC	50.0	55.0	58.2	57.6	878 bp	1.0	75.5