Hotspot

rps16

LSC · 4693-5749 · 1057 bp

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Pi 0

MarkerSeek score 40.00

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1057
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TAGATCCCAGCCATACCCAC	CACCACCCATATGCACGTAA	55.0	50.0	58.3	58.0	1190 bp	1.0	77.6
rps16_p2	CAGCCATACCCACACTCTTT	CACCACCCATATGCACGTAA	50.0	50.0	57.5	58.0	1183 bp	1.0	75.8
rps16_p3	TTAGATCCCAGCCATACCCA	CACCACCCATATGCACGTAA	50.0	50.0	57.5	58.0	1191 bp	1.0	75.7
rps16_p4	TAGATCCCAGCCATACCCAC	CGGAGTACTGACCAAACCAA	55.0	50.0	58.3	57.5	1150 bp	1.0	73.5
rps16_p5	TAGATCCCAGCCATACCCAC	TTTCACCACCCATATGCACG	55.0	50.0	58.3	58.5	1193 bp	1.0	73.5