Hotspot

psbA

LSC · 229-1290 · 1062 bp

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Pi 0

MarkerSeek score 40.00

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	ACCCATTTCTTGGTTATTTCTCCT	ATTTGGTTGCTTGGGAGTCC	37.5	50.0	58.1	58.4	1172 bp	1.0	44.3
psbA_p2	ACCCATTTCTTGGTTATTTCTCC	ATTTGGTTGCTTGGGAGTCC	39.1	50.0	56.7	58.4	1172 bp	1.0	43.0
psbA_p3	CCCATTTCTTGGTTATTTCTCCT	ATTTGGTTGCTTGGGAGTCC	39.1	50.0	56.5	58.4	1171 bp	1.0	40.9
psbA_p4	AACCCATTTCTTGGTTATTTCTCC	ATTTGGTTGCTTGGGAGTCC	37.5	50.0	57.4	58.4	1173 bp	1.0	40.2
psbA_p5	ACCCATTTCTTGGTTATTTCTCCT	TTTGGTTGCTTGGGAGTCC	37.5	52.6	58.1	57.9	1171 bp	1.0	40.0