Hotspot

matK

LSC · 1808-3310 · 1503 bp

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Pi 0

MarkerSeek score 40.00

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1503
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	AGTGATTTGATTCAACACCAATGT	ACCTATCTCCGGGGTATTTT	33.3	45.0	57.6	55.3	1720 bp	1.0	40.0
matK_p2	AGTGATTTGATTCAACACCAATGT	ACCTATCTCCGGGGTATTTTT	33.3	42.9	57.6	56.1	1720 bp	1.0	40.0
matK_p3	GTGATTTGATTCAACACCAATGT	ACCTATCTCCGGGGTATTTT	34.8	45.0	56.2	55.3	1719 bp	1.0	40.0
matK_p4	GTGATTTGATTCAACACCAATGT	ACCTATCTCCGGGGTATTTTT	34.8	42.9	56.2	56.1	1719 bp	1.0	40.0
matK_p5	AGTGATTTGATTCAACACCAATG	ACCTATCTCCGGGGTATTTT	34.8	45.0	56.0	55.3	1720 bp	1.0	40.0