Hotspot

atpI

LSC · 14504-15238 · 735 bp

Back to Jovibarba

Pi 0
MarkerSeek score 40.00
Primer available yes
Label atpI

Feature Metadata

label_name
atpI
feature_type
gene
region
LSC
length_bp
735
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpI_p1 AAGCTCATGCAATTCCCATT GTTCTATTTCTGTCAGAGGGCA 40.0 45.5 55.9 57.8 864 bp 1.0 45.9
atpI_p2 AGCTCATGCAATTCCCATTT GTTCTATTTCTGTCAGAGGGCA 40.0 45.5 55.9 57.8 863 bp 1.0 45.9
atpI_p3 AAGCTCATGCAATTCCCATTT GTTCTATTTCTGTCAGAGGGCA 38.1 45.5 56.7 57.8 864 bp 1.0 44.1
atpI_p4 AGCTCATGCAATTCCCATTTT GTTCTATTTCTGTCAGAGGGCA 38.1 45.5 56.7 57.8 863 bp 1.0 44.1
atpI_p5 AGCTCATGCAATTCCCATTT TCTATTTCTGTCAGAGGGCA 40.0 45.0 55.9 55.6 861 bp 1.0 44.0