Hotspot

atpA

LSC · 10320-11843 · 1524 bp

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Pi 0

MarkerSeek score 40.00

Primer available yes

Label atpA

Feature Metadata

label_name: atpA
feature_type: gene
region: LSC
length_bp: 1524
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA_p1	GGTTCAAATCCTATTGGACGC	TGTTTGGGGCGATGAAAGAA	47.6	45.0	57.3	58.0	1694 bp	1.0	66.4
atpA_p2	GGTTCAAATCCTATTGGACGC	TTACGTACGATCAGCGCAAA	47.6	45.0	57.3	58.0	1722 bp	1.0	66.3
atpA_p3	GGTTCAAATCCTATTGGACGC	ACGTACGATCAGCGCAAATA	47.6	45.0	57.3	57.8	1720 bp	1.0	64.9
atpA_p4	GGTTCAAATCCTATTGGACGC	TACGTACGATCAGCGCAAAT	47.6	45.0	57.3	57.8	1721 bp	1.0	64.9
atpA_p5	GGTTCAAATCCTATTGGACGC	ATGTTTGGGGCGATGAAAGA	47.6	45.0	57.3	57.8	1695 bp	1.0	64.7