Hotspot

atpH-atpI

LSC · 13824-14503 · 680 bp

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Pi 0

MarkerSeek score 40.00

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 680
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	CAGCAGAGACCAGTGGATTC	TTTTGCAACTTTAGCTGCGG	55.0	45.0	58.0	57.9	757 bp	1.0	78.8
atpH-atpI_p2	CAGCAGAGACCAGTGGATTC	TAGGAGAATCGATGGAGGGC	55.0	55.0	58.0	58.4	731 bp	1.0	76.9
atpH-atpI_p3	TCCAGCAGCAATAACGGAAG	TTTTGCAACTTTAGCTGCGG	50.0	45.0	58.3	57.9	777 bp	1.0	76.8
atpH-atpI_p4	AATAGAAGCAAGTCCGACGG	TTTTGCAACTTTAGCTGCGG	50.0	45.0	57.7	57.9	801 bp	1.0	76.6
atpH-atpI_p5	ATAACGGAAGCAGCAGAGAC	TTTTGCAACTTTAGCTGCGG	50.0	45.0	57.7	57.9	767 bp	1.0	76.5