Hotspot

trnK-UUU-rps16

LSC · 4036-4692 · 657 bp

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Pi 0
MarkerSeek score 40.00
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
657
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CGAATCCCTTGCTTCATCCA TTGAAAAGGGGGCTAAACCG 50.0 50.0 58.0 58.4 840 bp 1.0 76.6
trnK-UUU-rps16_p2 ACGAATCCCTTGCTTCATCC TTGAAAAGGGGGCTAAACCG 50.0 50.0 58.0 58.4 841 bp 1.0 76.6
trnK-UUU-rps16_p3 GGACGAATCCCTTGCTTCAT TTGAAAAGGGGGCTAAACCG 50.0 50.0 58.0 58.4 843 bp 1.0 76.6
trnK-UUU-rps16_p4 CGTACAAACTCTACCGACGG TTGAAAAGGGGGCTAAACCG 55.0 50.0 58.1 58.4 866 bp 1.0 76.1
trnK-UUU-rps16_p5 TCAGTCGTGGTCGTACAAAC TTGAAAAGGGGGCTAAACCG 50.0 50.0 57.9 58.4 877 bp 1.0 76.0