Hotspot

rpoC2

LSC · 350-7084 · 6735 bp

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Pi 0.471
MarkerSeek score 72.30
Primer available yes
Label rpoC2

Feature Metadata

label_name
rpoC2
feature_type
gene
region
LSC
length_bp
6735
variable_sites
2441
pi
0.471
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.77
markerseek_score
72.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoC2_p1 AAGCATTGCACACGTTAACG GCTGGGTACTTGACTCGAAG 45.0 55.0 58.0 58.0 1519 bp 0.5 59.7
rpoC2_p2 AAGCATTGCACACGTTAACG AGGGGCTCAAATGCTTCAAA 45.0 45.0 58.0 58.0 907 bp 0.5 59.6
rpoC2_p3 AAGCATTGCACACGTTAACG ATTGGAGAACCTGGGACTCA 45.0 50.0 58.0 58.0 1129 bp 0.5 59.4
rpoC2_p4 AAGCATTGCACACGTTAACG TGGAGAACCTGGGACTCAAT 45.0 50.0 58.0 58.0 1127 bp 0.5 59.4
rpoC2_p5 AAGCATTGCACACGTTAACG TATGGGTGAAGTCTGGCTGA 45.0 50.0 58.0 58.0 1201 bp 0.5 59.4