Hotspot

clpP

SSC · 129305-130885 · 1581 bp

Back to Jenufa

Pi 0.436
MarkerSeek score 83.40
Primer available yes
Label clpP

Feature Metadata

label_name
clpP
feature_type
gene
region
SSC
length_bp
1581
variable_sites
655
pi
0.436
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP_p1 CCGAGTGGTTTAAGGTACGG GCATGACCTCTTAACGTCGT 55.0 50.0 58.0 58.0 2110 bp 0.5 59.9
clpP_p2 ATGCTTGACCTCCACACAAG GCATGACCTCTTAACGTCGT 50.0 50.0 58.1 58.0 2479 bp 0.5 59.2
clpP_p3 AAAGGAAAGATGGCCGAGTG GCATGACCTCTTAACGTCGT 50.0 50.0 58.2 58.0 2123 bp 0.5 58.5
clpP_p4 TAATACCCCCTGCTCACCTC GCATGACCTCTTAACGTCGT 55.0 50.0 58.2 58.0 1947 bp 0.5 58.4
clpP_p5 CCGAGTGGTTTAAGGTACGG CCCCGGGGGTATAAAAACAC 55.0 55.0 58.0 58.2 2159 bp 0.5 58.1