Hotspot

chlL

LSC · 63273-64163 · 891 bp

Pi 0.198

MarkerSeek score 83.40

Primer available yes

Label chlL

Feature Metadata

label_name: chlL
feature_type: gene
region: LSC
length_bp: 891
variable_sites: 166
pi: 0.198
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlL_p1	TGGCTTCTGCGAAAAAGCTA	GGGCCTATCTGTTACACGTG	45.0	55.0	58.1	58.1	1782 bp	0.5	58.6
chlL_p2	TGGCTTCTGCGAAAAAGCTA	GCACAGGCCAAAAGGTAAAC	45.0	50.0	58.1	57.9	1858 bp	0.5	57.9
chlL_p3	AAAAGGCAAAGGCTAGCGTA	GGGCCTATCTGTTACACGTG	45.0	55.0	57.8	58.1	1854 bp	0.5	57.9
chlL_p4	AAAGGCAAAGGCTAGCGTAA	GGGCCTATCTGTTACACGTG	45.0	55.0	57.8	58.1	1853 bp	0.5	57.9
chlL_p5	AAGGCAAAGGCTAGCGTAAA	GGGCCTATCTGTTACACGTG	45.0	55.0	57.8	58.1	1852 bp	0.5	57.9