Hotspot

cemA

LSC · 83553-84764 · 1212 bp

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Pi 0.24
MarkerSeek score 84.10
Primer available yes
Label cemA

Feature Metadata

label_name
cemA
feature_type
gene
region
LSC
length_bp
1212
variable_sites
274
pi
0.24
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
84.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
cemA_p1 AAGGCCAAAAGGTGAACGAT TTCATGCGCTATCAACTGCT 45.0 45.0 58.0 58.0 2482 bp 0.5 59.7
cemA_p2 AGGCCAAAAGGTGAACGATT TTCATGCGCTATCAACTGCT 45.0 45.0 58.0 58.0 2481 bp 0.5 59.7
cemA_p3 AATGACCCAGCACGTAATCC TTCATGCGCTATCAACTGCT 50.0 45.0 58.0 58.0 2299 bp 0.5 59.5
cemA_p4 AAGGCCAAAAGGTGAACGAT TTTTTCTCCCCAATCAGCCC 45.0 50.0 58.0 58.1 2062 bp 0.5 59.5
cemA_p5 AGGCCAAAAGGTGAACGATT TTTTTCTCCCCAATCAGCCC 45.0 50.0 58.0 58.1 2061 bp 0.5 59.5