Hotspot

orf579-trnG(gcc)

SSC · 125643-127068 · 1426 bp

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Pi 0.419
MarkerSeek score 83.30
Primer available yes
Label orf579-trnG(gcc)

Feature Metadata

label_name
orf579-trnG(gcc)
feature_type
igs
region
SSC
length_bp
1426
variable_sites
569
pi
0.419
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
orf579-trnG(gcc)_p1 AGACCAAACCCCATTCAACC TAAACCGTGTTGACCTGGTG 50.0 50.0 58.0 58.0 2415 bp 0.5 59.6
orf579-trnG(gcc)_p2 TCGACAAAATCAACGTTGCG TAAACCGTGTTGACCTGGTG 45.0 50.0 58.0 58.0 2325 bp 0.5 59.6
orf579-trnG(gcc)_p3 CGAAGTGGCAAATTGCGAAT TAAACCGTGTTGACCTGGTG 45.0 50.0 58.0 58.0 2603 bp 0.5 59.5
orf579-trnG(gcc)_p4 AGACCAAACCCCATTCAACC AAAGTTTGCTACACGACCGT 50.0 45.0 58.0 58.1 1806 bp 0.5 59.5
orf579-trnG(gcc)_p5 TCGACAAAATCAACGTTGCG AAAGTTTGCTACACGACCGT 45.0 45.0 58.0 58.1 1716 bp 0.5 59.5