Hotspot

orf333-orf579

SSC · 122553-123902 · 1350 bp

Back to Jenufa

Pi 0.488
MarkerSeek score 83.40
Primer available yes
Label orf333-orf579

Feature Metadata

label_name
orf333-orf579
feature_type
igs
region
SSC
length_bp
1350
variable_sites
622
pi
0.488
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
orf333-orf579_p1 AATTTCAGCTCACGGAGACC CAAGTGCACTTCTTCCTGGT 50.0 50.0 57.9 58.0 2364 bp 0.5 59.0
orf333-orf579_p2 AATTTCAGCTCACGGAGACC TTTAGCGCCGGAATGATTGA 50.0 45.0 57.9 58.0 2057 bp 0.5 58.9
orf333-orf579_p3 AATTTCAGCTCACGGAGACC TGCCTCCCATTCAGGTTCTA 50.0 50.0 57.9 58.0 2315 bp 0.5 58.9
orf333-orf579_p4 AATTTCAGCTCACGGAGACC CAGTTGCGAGCGTCATTTTT 50.0 45.0 57.9 58.0 2163 bp 0.5 58.9
orf333-orf579_p5 AATTTCAGCTCACGGAGACC AGTTTAGCGCCGGAATGATT 50.0 45.0 57.9 58.0 2059 bp 0.5 58.9