Hotspot

trnW(cca)-atpA

LSC · 79772-80492 · 721 bp

Back to Jenufa

Pi 0.413
MarkerSeek score 83.00
Primer available yes
Label trnW(cca)-atpA

Feature Metadata

label_name
trnW(cca)-atpA
feature_type
igs
region
LSC
length_bp
721
variable_sites
274
pi
0.413
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
83.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnW(cca)-atpA_p1 AGGTGGGTTTTTAACGTCCC GTAGCTCCAGTGTAAGGTGC 50.0 55.0 58.0 58.0 1885 bp 0.5 59.8
trnW(cca)-atpA_p2 AGGTGGGTTTTTAACGTCCC ATCAACTGGGCGAGCTAAAG 50.0 50.0 58.0 58.0 1502 bp 0.5 59.6
trnW(cca)-atpA_p3 AGGTGGGTTTTTAACGTCCC ATACAGAACGACGCGAAACA 50.0 45.0 58.0 57.9 1581 bp 0.5 59.4
trnW(cca)-atpA_p4 TTATCATTCCCGCGACACAG GTAGCTCCAGTGTAAGGTGC 50.0 55.0 58.1 58.0 2151 bp 0.5 59.4
trnW(cca)-atpA_p5 GTCGTGGGTTCAAGTCCTAC GTAGCTCCAGTGTAAGGTGC 55.0 55.0 57.9 58.0 1483 bp 0.5 59.3