Hotspot

psbF-rpoBa

LSC · 15185-17258 · 2074 bp

Back to Jenufa

Pi 0.49
MarkerSeek score 75.40
Primer available yes
Label psbF-rpoBa

Feature Metadata

label_name
psbF-rpoBa
feature_type
igs
region
LSC
length_bp
2074
variable_sites
697
pi
0.49
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.69
markerseek_score
75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbF-rpoBa_p1 GGCTGGCATATCGTTAACCA AGCTCCCCCGTTCAGTATAA 50.0 50.0 58.0 57.8 2908 bp 0.5 58.3
psbF-rpoBa_p2 TGGCTGGCATATCGTTAACC AGCTCCCCCGTTCAGTATAA 50.0 50.0 58.0 57.8 2909 bp 0.5 58.3
psbF-rpoBa_p3 GGAGTTTTTCTTTGGCTGGC AGCTCCCCCGTTCAGTATAA 50.0 50.0 57.8 57.8 2921 bp 0.5 57.4
psbF-rpoBa_p4 GGCTGGCATATCGTTAACCA CTAGCTCCCCCGTTCAGTAT 50.0 55.0 58.0 58.3 2910 bp 0.5 57.3
psbF-rpoBa_p5 TGGCTGGCATATCGTTAACC CTAGCTCCCCCGTTCAGTAT 50.0 55.0 58.0 58.3 2911 bp 0.5 57.3