Hotspot

rpl16

LSC · 88089-89891 · 1803 bp

Back to Jatropha

Pi 0.0177
MarkerSeek score 58.80
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1803
variable_sites
31
pi
0.0177
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 GGCATCCCCAAGGTCTTTTT ATTGAATAGACCCGCGGTTC 50.0 50.0 58.4 58.1 1868-1909 bp 1.0 80.4
rpl16_p2 GGCATCCCCAAGGTCTTTTT GATTGAATAGACCCGCGGTT 50.0 50.0 58.4 58.1 1869-1910 bp 1.0 80.4
rpl16_p3 GGCATCCCCAAGGTCTTTTT CGCGGTTCGATTGATGTAGT 50.0 50.0 58.4 58.4 1856-1897 bp 1.0 77.2
rpl16_p4 GCATCCCCAAGGTCTTTTTC ATTGAATAGACCCGCGGTTC 50.0 50.0 57.0 58.1 1867-1908 bp 1.0 74.9
rpl16_p5 GCATCCCCAAGGTCTTTTTC GATTGAATAGACCCGCGGTT 50.0 50.0 57.0 58.1 1868-1909 bp 1.0 74.9