Hotspot

rpl32-trnL-UAG

SSC · 122125-123143 · 1019 bp

Back to Jatropha

Pi 0.0353
MarkerSeek score 76.70
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1019
variable_sites
31
pi
0.0353
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.86
markerseek_score
76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GCGAAATCCCTTTCTACAGGT AGCGTGTCTACCGATTTCAC 47.6 50.0 58.0 58.0 962-1098 bp 1.0 78.2
rpl32-trnL-UAG_p2 GCGAAATCCCTTTCTACAGGT CAGCGTGTCTACCGATTTCA 47.6 50.0 58.0 58.0 963-1099 bp 1.0 78.2
rpl32-trnL-UAG_p3 GCGAAATCCCTTTCTACAGGT TCCTAAGAGCAGCGTGTCTA 47.6 50.0 58.0 57.9 972-1108 bp 1.0 77.3
rpl32-trnL-UAG_p4 GCGAAATCCCTTTCTACAGGT CCTAAGAGCAGCGTGTCTAC 47.6 55.0 58.0 57.8 971-1107 bp 1.0 76.7
rpl32-trnL-UAG_p5 GCGAAATCCCTTTCTACAGGT CTAAGAGCAGCGTGTCTACC 47.6 55.0 58.0 57.8 970-1106 bp 1.0 76.7