Hotspot

clpP-psbB

LSC · 78398-78921 · 524 bp

Back to Jatropha

Pi 0.0268

MarkerSeek score 70.80

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 524
variable_sites: 14
pi: 0.0268
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	TGAATCGTCTTCCCCAGGAT	CGATACCAAGGCAAACCCAT	50.0	50.0	58.1	58.2	598-605 bp	1.0	79.3
clpP-psbB_p2	TGAATCGTCTTCCCCAGGAT	CAAACGACCGGGATCATTCA	50.0	50.0	58.1	58.3	635-642 bp	1.0	78.9
clpP-psbB_p3	TTGAATCGTCTTCCCCAGGA	CGATACCAAGGCAAACCCAT	50.0	50.0	58.3	58.2	599-606 bp	1.0	77.4
clpP-psbB_p4	TGAATCGTCTTCCCCAGGAT	ACACGATACCAAGGCAAACC	50.0	50.0	58.1	58.5	601-608 bp	1.0	77.4
clpP-psbB_p5	TGAATCGTCTTCCCCAGGAT	AAACGACCGGGATCATTCAA	50.0	45.0	58.1	57.5	634-641 bp	1.0	77.2