Hotspot

rps12-clpP

LSC · 76089-76290 · 202 bp

Back to Jatropha

Pi 0.0396

MarkerSeek score 70.80

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: LSC
length_bp: 202
variable_sites: 8
pi: 0.0396
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	GCGGGGGATTTAGTGACATT	TATGTCAGCAACAGAAGCCC	50.0	50.0	57.9	57.9	328-368 bp	1.0	80.5
rps12-clpP_p2	GCGGGGGATTTAGTGACATT	GCACAAAGAACGGGAAAACC	50.0	50.0	57.9	58.2	387-427 bp	1.0	79.9
rps12-clpP_p3	CTAGTACATGTTCCTCGGCG	TATGTCAGCAACAGAAGCCC	55.0	50.0	57.9	57.9	367-407 bp	1.0	79.8
rps12-clpP_p4	CTAGTACATGTTCCTCGGCG	GCACAAAGAACGGGAAAACC	55.0	50.0	57.9	58.2	426-466 bp	1.0	79.2
rps12-clpP_p5	CAAGAGCGGGGGATTTAGTG	TATGTCAGCAACAGAAGCCC	55.0	50.0	58.3	57.9	333-373 bp	1.0	78.2